Fig. 2
From: Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice
Expression of the Sc alleles. a Expression of Sc-i and Sc-j in the developing anthers of E5, T65, and their F1 by qRT-PCR using a common primer pair (P3/P8, Supplementary Fig. 1) for all the alleles/Sc-i paralogs (except for Sc-is). The values (means of three biological replicates with s.e.m.) were normalized to OsActin 1 transcript levels. b qRT-PCR analysis of Sc-j (using P3/P8) in separated pollen and anther wall cells (at early-bicellular stage) of T65. Data are shown as means ± s.e.m. (n = 3). c Different Sc protein abundances in anthers (at stages of uninucleate late-microspore to early-bicellular pollen) of the parents and the F1, detected with anti-Sc antibodies
