Fig. 3
From: Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice
Functional analysis of Sc-j. a qRT-PCR analysis of relative Sc-j expression levels (using the Sc-j-specific primers P1/P2) in the anthers and isolated pollen (at early-bicellular stage) of the F1 (T65/E5) and T65, calculated as the ratios of the transcript per copy Sc-j to the transcript of OsActin 1 (two copies), because there is only one Sc-j copy in the F1. The data are shown as means ± s.e.m. (n = 3), and * and ** indicate significance at P < 0.05 and P < 0.01, respectively, by two-tailed Student’s t test (the same for Fig. 3c). b Percentages of cDNA clones from Sc-j, Sc-ia, and Sc-ib (Sc-ib1/ib2) of the F1 anthers of two stages indicated. c Expression of Sc-j in the early-bicellular stage anthers of two T1 (T65 background) plants carrying the hemizygous antisense-transgene of Sc-j. The detected transcript levels in Anti-1 and Anti-2 could be mainly from the pollen grains (~ 50%) without the antisense-transgene. d Semi-sterility of three T1 plants carrying the hemizygous antisense-transgene. WT (T-), a T1 segregant without the antisense-transgene. Scale bars, 50 μm
