Fig. 4
From: Genomic structural variation-mediated allelic suppression causes hybrid male sterility in rice
Genomic editing of the Sc-i paralogs rescues Sc-j expression and male fertility in the hybrids. a Targeted editing of the sites (arrowed) in E5 produced a plant (E5-d1) with deletion of one of the ~ 28-kb segments (Supplementary Fig. 6), and editing another site (underlined) specific to Sc-ib1 and Sc-ib2 of E5 identified two plants (E5-ed1 and E5-ed2) with mutated Sc-ib1 and Sc-ib2 and intact Sc-ia (Supplementary Fig. 10). The SNP (blue) in Sc-ib1 and Sc-ib2 formed the PAM (NGG) required for genome editing. Primers P9a-P9e combined with the Sc-i-specific P10 were used for qRT-PCR of the intact and mutated Sc-i transcripts show in (c). b Improvement of pollen fertility (sterile pollen grains arrowed) in the edited F1 plants from crossing E5-d1, E5-ed1, and E5-ed2 with T65. Scale bars, 50 μm. c Expression levels (in the meiocyte stage anthers) of the Sc-i paralogs in the F1 (T65/E5), and the intact Sc-ia and mutated Sc-ib1/Sc-ib2 in the edited F1 plants. Data are shown as means ± s.e.m. (n = 3). d Expression levels of per copy Sc-j (qRT-PCR using P1/P2) in the early-bicellular stage anthers of the intact and edited F1 plants and T65. The data are shown as means ± s.e.m. (n = 3), and * and ** indicate significance at P < 0.05 and P < 0.01, respectively, by two-tailed Student’s t test
