Fig. 6

Comparison of exemplary electron density maps of phycocyanin (2mFo − DFc at 1.5σ level) showing one of the phycocyanobilin molecules present in the structure (left) and a view along residues 90–95 of α-helix 6 (right) obtained by pink-beam serial crystallography at a synchrotron (a–c) and with serial femtosecond crystallography (d). a The electron density of dataset Phyco_A1 obtained from the measurement of 45 diffraction images using the APS single bunch. b The electron density of dataset Phyco_B obtained from the measurement of 205 diffraction images using the APS single bunch. c The same regions obtained from 52 diffraction images using a longer exposure time of 3.68 μs per crystal. d Structure 4ZIZ from the PDB based on 6679 single shot diffraction images collected at LCLS