Fig. 1
From: Systematic proteome and proteostasis profiling in human Trisomy 21 fibroblast cells
Proteome and proteostasis investigation on T21 fibroblast cells. a Fibroblast cells collected from a pair of monozygotic (MZ) twins discordant for Trisomy 21 (T21), together with 11 unrelated T21 individuals bearing Down syndrome (DS) and 11 unrelated controls were used for analyzing the effect of T21. T1DS and T2N denote the T21 and normal twin used. b A reproducible and accurate data-independent acquisition mass spectrometry (DIA-MS) was used in conjunction with a massively parallel targeted data analysis strategy of SWATH-MS to profile the proteomes. c A dimethylation labeling-based shotgun proteomics was used to measure protein abundance change in T21 and N sample mixtures. SAX strong anion exchanger. d A pulse SILAC (pSILAC) experiment determines the protein degradation and turnover rates in T1DS and T2N, respectively, across 6 time points over 24 h
