Fig. 5

Extent of protein degradation reveals organelle-related gradients in T21. a Selected significant, overlapping biological processes after GESA analysis at mRNA, protein, and K _deg levels. The logarithm odds ratios (LOR) were visualized on a red–blue color scale, with blue color denoting LOR > 0 (i.e., T1DS gene products are under represented) and red denoting LOR < 0 (i.e., T1DS products are over-represented). b The five classes of proteins based on K _deg regulation extent. The red arrow denotes zero K _deg regulation. c The ranked list of protein degradation regulation under T21 stress was divided into five segments of proteins for which GO biological processes enriched in each segment were selected and displayed. d Cellular components annotation and enrichment analysis indicates the extent to which protein degradation forms an organelle-related gradient in T21. e The transcript, protein, protein degradation, and protein synthesis FC of all the proteins annotated in each organelle were averaged for hierarchical clustering analysis. f Comparing the number of proteins, absolute protein copies, protein investment, and synthesis expense for different organelles in T1DS and T2N, respectively. K _deg protein degradation rate, K _syn synthesis rate