Fig. 3
RNA-seq identifies KSRP regulated primary miRNA. a Microscopy images of THP-1 cells transduced with lentiviruses expressing GFP (Con) or KSRP (KSRP). The GFP+ cells were subsequently sorted by FACS. The bar represents 50 μm. b Volcano plots displaying the average log2 ratios of the expression of all pri-miRNAs in poly(A)-enriched RNA-seq data and the expression of mature miRNAs in small RNA-seq data from KSRP versus Con groups obtained in a. miRNA candidates whose processing was either promoted (upper) or inhibited (lower) by KSRP were shown in multi-color. miRNAs with statistically significant (p-value < 0.05, pairwise multiple comparisons) are shown in red. c Heat map representation of relative pri-miRNA and mature miRNA expression levels obtained from the RNA-seq data for the 16 selected miRNAs. d, e THP-1 cells in which endogenous KSRP was knocked down or overexpressed were analyzed by qPCR for KSRP expression d or the primary and mature transcripts expression corresponding to the 16 miRNAs e. Three technical replicates from a single experiment. Data are shown as means±s.d. **P < 0.01, ***P < 0.001, Student’s t-test
