Fig. 7
KSRP promotes the processing of pri-129-1 in vivo. a Schematic representation of the in vivo processing assay performed by co-transfecting GFP-labeled pri-129-1 (129_WT) or GFP-labeled KSRP-binding site mutant (129_Mut1, 2, 3, 4 for each site and 129_MutF for all four sites) constructs with the KSRP construct or empty vector. The mutant sequences in the constructs are shown in the right panel. b Images of GFP and RFP fluorescence in 293 T cells used in the in vivo processing assay, as indicated in h (Scale bar, 100 μm). RFP fluorescence from a co-transfected plasmid was used as control for transfection efficiency. c The quantitative ratio of GFP expression to RFP expression in b. d Schematic representation of the in vivo processing assay in zebrafish, as described in the Methods. e, f Images of GFP fluorescence in the 4 h.p.f. e or 24 h.p.f. f zebrafish embryos at after the zebrafish zygote was microinjected with a combination of GFP-pri-129_WT or GFP-pri-129_MutF fusion mRNAs with the KSRP mRNA or the control (Scale bar, 200 μm). Data are shown as means±s.d. *P < 0.05, **P < 0.01, Student’s t-test
