Table 3 Mixed linear regression model estimates for ACTH concentrations (ng/ml) measured in diluted medium samples from cells transfected with KCNQ1 and KCNE2

From: Two missense mutations in KCNQ1 cause pituitary hormone deficiency and maternally inherited gingival fibromatosis

Parameter

Estimate

SE

P

Intercept (WT KCNQ1/KCNE2)a

9.59

0.84

<0.001

Time

0.62

0.15

<0.001

KCNQ1-Arg116Leu/KCNE2

−1.88

0.42

<0.001

KCNQ1-Pro369Leu/KCNE2

−1.29

0.42

0.004

KCNQ1-Gly589Asp/KCNE2

−0.77

0.42

0.075

  1. Mixed linear regression model estimates for ACTH concentrations (ng/ml) measured in diluted medium samples (1:10) in the reference condition (WT KCNQ1/KCNE2) and three other KCNQ1 conditions (KCNQ1-Arg116Leu/KCNE2, KCNQ1-Pro369Leu/KCNE2, KCNQ1-Gly589Asp/KCNE2), their standard errors (SE), and corresponding P values. The model included both the growth time (per 1 h) and the KCNQ1/KCNE2 conditions as fixed effects and the experiment number as a random intercept. Based on the regression model, the KCNQ1-Arg116Leu with KCNE2 produced 20% (−2 h) or 17% (0 h) less ACTH than the reference (WT KCNQ1 with KCNE2) (P < 0.001). Similarly, the KCNQ1-Pro369Leu with KCNE2 produced approximately ~12–13% less ACTH (P = 0.004) than the reference environment. ACTH levels produced by the cells transfected with the LQT1 mutant KCNQ1-Gly589Asp/KCNE2, which served as a control, did not differ significantly from the reference environment. The CRF-induced ACTH responses between the four KCNQ1/KCNE2 conditions did not differ (data not shown)
  2. aAt −2 h
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