Fig. 4
From: Self-assembling dipeptide antibacterial nanostructures with membrane disrupting activity
The diphenylalanine nanostructures are non-hemolytic and non-cytotoxic toward human cell lines. a, b MTT cell viability analysis. The cytotoxicity of the diphenylalanine nanostructures toward two human cell lines was evaluated by the MTT assay using the NAD(P)H-dependent Tetrazolium dye following over-night treatment with the indicated compounds. a HEK 293 embryonic kidney cell line. b HaCaT keratinocyte cell line. c, d Hemolysis analysis. The hemolytic activity of the diphenylalanine nanostructures was assessed by incubating defibrinated sheep blood with the compounds and calculation of the subsequent hemolysis from absorbance readings at 451 and 405 nm. The percentage of non-disrupted blood cells is presented c, as well as the treated pelleted erythrocytes d
