Fig. 1

CBX6-depleted ESCs spontaneously differentiate. a Phase contrast image of shCtl, shCbx6, and shCbx7 ESC lines. b Accumulative proliferation measurements of shCtl and shCbx6 along passages. SEM, standard error of mean; P, passage; Signif., significance. Significance was analyzed through Student’s t test. Significance was considered when P value was ≤0,05. P value of ** is ≤10⁻², P value of *** is ≤10⁻³. c RT-qPCR analysis of control and CBX6-depleted ESCs. Results are shown relative to shCtl and are normalized to the housekeeping gene Rpo. Error bars represent standard deviation (SD) of three independent experiments. Significance was analyzed through Student’s t test. Significance was considered when P value was ≤0.05 (*). d Phase contrast image of AP staining performed on shCtl or shCbx6 ESCs (right panel); quantification of the AP staining assays, representing the mean of three independent experiments in which around 40 random colonies were counted (left panel)