Fig. 3

CBX6 interactome. a Protein complex affinity capture workflow. b Statistically enriched proteins in the 3×FLAG IP identified by permutation-based FDR-corrected t-test. The plot shows log₂ (difference) ratios of averaged protein intensities of the 3×FLAG pull-down over the control, plotted against the –log₁₀ (P value). The hyperbolic significance curve was calculated based on a combination of P value and fold-change. The proteins in the upper right corner represent the bait and its interactors (marked in red). A fold-change of ≥2 and a false discovery rate (FDR) of 0.05 were considered significant. c Co-IPs from total ESC extracts using an antibody against 3×FLAG. Western blots of different proteins are shown. d Co-IPs from total CBX6-3×FLAG-expressing ESC extracts using an antibody against RING1B. Western blots of different proteins are shown