Fig. 4

CBX6 genome-wide distribution features. a TSS (±5 kb) enrichment plot of CBX6 ChIP-seq at 2730 CBX6 target sites. b ChIP-qPCR validation of target genes of CBX6 in control and CBX6-3×HA-expressing cells. Results are shown relative to the input percentage. Error bars represent SD of three biological replicates. Significance was analyzed through Student’s t test. Significance was considered when P value was ≤0.05 (*). c Box plots showing expression of CBX6, H3K27me3, and H3K36me3 target genes. d GO analysis of biological functions and signaling pathways of CBX6 target genes. P values are plotted in −log. e RNAseq heat map of up- and downregulated genes in CBX6-depleted cells as compared to control cells. Only genes up- or downregulated by at least 1.5-fold as compared to control cells are shown. f Venn diagrams showing the overlap between CBX6 and CBX7 deregulated genes upon their depletion