Fig. 5

CBX6 occupies cPRC1 sites. a TSS (±5 kb) enrichment plot of CBX6, RING1B, CBX7, H2AK119ub, and SUZ12 ChIP-seq at 2730 CBX6 target sites. b Venn diagrams showing the overlap of CBX6 target genes with those of RING1B CBX7, H2AK119ub¹³, SUZ12¹³, and PCGF6³⁵. c (left) TSS (±5 kb) enrichment plot of RING1B in shCtl and shCbx6 ESCs, at 2730 CBX6 target sites. (right) ChIP-qPCR in shCTL or shCBX6 ESCs of RING1B and H2AK119Ub. d (left) TSS (±5 kb) enrichment plot of CBX6 in shCtl and shCbx7 ESCs, at 2730 CBX6 target sites. (right) ChIP-qPCR in shCTL and shCBX6 (or shCbx7) ESCs of CBX7 (or CBX6), respectively. e ChIP-qPCR in shCTL or shCBX6 ESCs of Suz12 and H3K27me3. f ChIP-qPCR of CBX6 in shCTL and shSuz12 ESCs. c–f For all the experiments, an intergenic region was used as a negative control gene. Results are shown relative to percentage of input. For all the experiments an intergenic region was used as a negative control gene. Results are shown relative to percentage of input. Error bars represent SD of three biological replicates. Error bars represent SD of three biological replicates