Fig. 3
From: Injury-activated glial cells promote wound healing of the adult skin in mice
Expression profile analysis of glial lineage upon skin injury. a Experimental design for skin sample collection of Ctrl and D7-injured Dhh-Cre; tdTomato animals. Single cell suspensions were sorted for tdTomato expression using a flow cytometer (0.8–1.8% of total cell fraction). Total RNA samples of gated cells were amplified and subsequently subjected to RNA-Seq using Illumina RNA-Seq platform. b Volcano plot of the gene expression profile of Dhh-Cre-traced tdTomato+ cells of intact skin and D7 post injury. Coloured data points meet the thresholds of FC above 1.5 and under −1.5, P-value < 0.05, FDR < 0.05. c Unsupervised sample clustering heat map of gene expression with a significantly differential expression. d Gene GO MetaCore prediction of the product localisation of the significantly downregulated (506 genes) and upregulated genes (526 genes) shows a significant enrichment of genes, the products of which are excreted. e Data representation for the percentage of gene products predicted to be destined to the extracellular space. f Volcano plot of the expression value of the genes with predicted extracellular localisation of their product. g Quantitative Venn diagram of the differentially expressed “secretome” and the associated biological function
