Fig. 5
From: Structural insights into DNA cleavage activation of CRISPR-Cas9 system
The MD models of the wild-type HNH and RuvC active sites. a Close-up view of the HNH active site in complex with the target strand and one Mg2+ ion. b Close-up view of the RuvC active site in complex with the non-target strand and two Mg2+ ions. c DNA cleavage activities of the wild-type SpCas9 and three mutants detected by agarose gel electrophoresis. d DNA cleavage activities detected by capillary electrophoresis. The reference is the uncut, 692-bp long DNA strands. Data are shown for one representative experiment from three independent experiments with similar results
