Fig. 5
Functional analysis of NOG1. a ECH enzymatic activity assays of NOG1/nog1 and 6×His fusion protein by eukaryotic expression in Pichia yeast. The empty pPICZ-C vector plasmid was transfected as control (pPICZ), and pPICZ-GC and pPICZ-S176 were transfected by NOG1 and nog1, respectively (n = 10). b Enzymatic activity assays of ECH in young panicles among Guichao 2, SIL176, and transgenic plants (n = 10). c Comparison of the total fatty acid content in young panicles among Guichao 2, SIL176, and transgenic plants (n = 3). d–f Comparison of JA, OPDA, and JA-Ile levels in young panicles among Guichao 2, SIL176, and transgenic plants (n = 10). g Main panicle of Guichao 2 and JA-treated Guichao 2. Scale bars, 5 cm. h, i Comparison of JA contents and the number of grains per main panicle between Guichao 2 and JA-treated Guichao 2 (n = 10). j Comparison of NOG1 expression levels between Guichao 2 and JA-treated Guichao 2 (n = 3). Data are means, with error bars showing SEM (two-tailed Student’s t test; **P < 0.01; *P < 0.05; NS, P > 0.05)
