Fig. 2
The RidL9-258 β-hairpin determines retromer binding of the fragment in cells. a HeLa cells were transiently transfected with vectors producing GFP-fusion proteins or GFP as indicated (green) and immuno-stained for the retromer (Vps26; red). Scale bar, 10 μm. b Co-localization of RidL variants with retromer (a) was scored. The Pearson’s correlation coefficient was calculated in three independent experiments with at least 50 cells each (mean and standard deviation, SD, of the mean from each experiment; one way ANOVA; ***P < 0.001)
