Fig. 7
D. discoideum TBC1D5 reduces Rab7 on LCVs. a Imaging flow cytometry (IFC) images of D. discoideum DH1 (WT or Δtbc1d5) producing GFP-Rab7 (pAW9), infected (MOI 5, 2 h) with mPlum-producing L. pneumophila JR32 (pAW14). b Quantification of IFC co-localization score between GFP and mPlum in >750 cells per sample at the time points p.i. indicated. The data show mean and 95% confidence intervals of one representative experiment out of four independent experiments (***P < 0.001; two-way ANOVA with Bonferroni post hoc test). c IFC images of D. discoideum DH1 producing GFP-Rab7 (pAW9), infected (MOI 5, 2 h) with DsRed-producing L. pneumophila JR32, ΔicmT or ΔridL harboring pCR77. d Quantification of IFC co-localization score between GFP and DsRed in >1000 cells per sample at the time points p.i. indicated. The data show mean and 95% confidence intervals of one representative experiment out of four independent experiments (***P < 0.001; two-way ANOVA with Bonferroni post hoc test)
