Table 2 SPR analysis of RidL–Vps29 interactions

From: Structural insights into Legionella RidL-Vps29 retromer subunit interaction reveal displacement of the regulator TBC1D5

Analyte

Mol. Mass (kDa)

Binding to

  

Vps29

Vps29L152E

RidL2–1167

132

Yes

No

RidL10–258

29

Yes

No

RidL259–1167

102

No

No

RidL2–1167-Δβ-hairpin

130

No

No

RidL2–1167-ΔF-loop

130

Yes

No

  1. For SPR measurements, biotinylated Vps29 or Vps29L152E were immobilized, and RidL variants were used as analytes. Due to limited signal saturation within the measured analyte concentration range, binding was assessed only qualitatively (Supplementary Fig. 4). The RidL β-hairpin and a Vps29 hydrophobic groove determine protein-protein interaction
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