Fig. 4
From: Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells
Engineered renal AML-like tumours derive from renal epithelial cells. a Fluorescence images of kidney tissue from doxycycline-treated Pax8-rtTA;TRE-LC1;R26R-td Tomato mice and from Sox10-Cre;R26R-td Tomato mice and respective controls. Scale bars: 50 μm. b Images of primary kidney cells isolated from doxycycline-treated Pax8-rtTA;TRE-LC1;R26R-td Tomato mice and from Sox10-Cre R26R-td Tomato mice. Scale bars: 50 μm. c Red fluorescence signals in spheres derived from infection of cultures in b with shRNA-Tsc2 + Cdkn2a lentivirus. Scale bars: 50 μm. d Differentiation of Pax8-td Tomato positive spheres into epithelial cells (E-CADHERIN immunofluorescence), adipocytes (Nile Red staining) and glial cells (GFAP immunofluorescence). tdTomato was detected using an anti-RFP antibody. Scale bars: 50 μm. e tdTomato-positive shRNA-Tsc2 + Cdkn2a allograft derived from Pax8-rtTA;TRE-LC1;R26R-td Tomato spheres. Scale bars: 1 cm. f Representative red fluorescence in histological sections of shRNA-Tsc2 + Cdkn2a allografts derived from Pax8-rtTA;TRE-LC1;R26R-td Tomato mice: DAPI and td Tomato overlay. Scale bars: 50 μm. Lower panels represent zooms of the boxed regions in the upper panels. g Example of second-generation shRNA-Tsc2 + Cdkn2a td Tomato positive sphere and allograft histological section derived from a primary shRNA-Tsc2 + Cdkn2a allograft that originated from a Pax8-rtTA;TRE-LC1;R26R-td Tomato mouse. Scale bars: 50 μm
