Fig. 1

Monocytic-MDSCs are accumulated in tumors following radiation in WT but not in CCR2^−/− mice. Tumors were harvested 3 days post-IR and subjected to flow cytometry analysis. a Gating strategy and b flow analysis of Ly6C^hi populations in control (NonIR) and irradiated (IR) tumors grown in WT or CCR2^−/− hosts (n = 4). b Left, cell percentage; Right, absolute cell number. ***P = 0.0001; *P < 0.05, as calculated by two-sided Student’s t-test. The experiments were repeated 3 times. Data are presented as mean ± s.e.m