Fig. 4
From: The low affinity neurotrophin receptor CD271 regulates phenotype switching in melanoma
CD271 transient overexpression in vitro reveals a reversible phenotype. a Schematic representation of the inducible vector (TetON) to overexpress CD271 in melanoma cell lines. The viral backbone carries the green fluorescent protein (GFP) and infrared fluorescent protein (iRFP) reporters under the SV40 and PGK promoters respectively, as well as the CD271 overexpression cassette (CMVTOCD271) or an empty vector (CMVTOEV) under the inducible CMVTetOperon (CMVTO) promoter. The overexpression of the gene is controlled by doxycycline and is based on the TRexTM system (pLenti CMV TetR Blast, Addgene no. 17492). b Crystal violet staining of adherent cells (upper panels) and suspension cells forced to reattach on fibronectin-coated plates (lower panels). Not treated (day 0), treated with doxycycline for 24 h (day 1), and subsequently released from doxycycline for 48 h (day 4). c Quantification of cell numbers of b (n = 3, P values > 0.05, ≤ 0.01). Error bars indicate S.D. All experiments done with cell line M010817
