Fig. 2
From: Discovery and characterization of stable and toxic Tau/phospholipid oligomeric complexes
Characterization of the Tau and K18 protein/phospholipid complexes. a Chromatograms of BPS vesicles alone (blue) and after incubation for 24 h with K18 (black) and Tau (red) at a molar protein:phospholipid ratio of 1:20. The samples were run on a Superose 6 column at a flow rate of 0.5 ml min−1. Vesicles eluted in the void volume (15 min), globular particles formed by Tau and K18 eluted between 20 and 25 min and between 27 and 32 min, respectively, and monomeric Tau and K18 eluted at 30 min and 36 min, respectively. b Negative-stain EM images of the void volume, the globular particles, and monomer fractions of Tau (top) and K18 (bottom). The scale bars are 100 nm. c SDS-PAGE gel of the void volume (V) and the peaks representing the protein/phospholipid complexes (C) and the monomers (M) obtained with Tau (top) and K18 (bottom). The asterisks mark bands of monomeric proteins and the dashed red rectangles indicate oligomeric species. The phospholipids, containing 1% NBD-labeled phospholipids, which were imaged separately with a Typhoon trio fluorescent scanner, are shown on the bottom (labeled fPS)
