Fig. 1
From: Thermostable exoshells fold and stabilize recombinant proteins
Engineering of six thermostable exoshell variants. a Overview of the encapsulation process, as generated using PyMOL v1.8.6.0. b Schematic of terminologies. (i) POI, protein of interest; (ii) thermostable exoshell-POI (tES-POI), POI fused to the C-terminus of a tES subunit; (iii) POI expressed in the presence of a tES shell but without encapsulation; (iv–vi) coexpression of tES-POI with tES(+), tES(−), and tES(+/−) shells; and (vii) tES(+)F116H, pH-titratable subunits. c Dynamic light scattering of tES variants. Mutation of F116 to H resulted in a pH-titratable assembly and dissociation. The results are expressed as means ± standard deviation (SD) (n = 3)
