Table 1 Sequences of various complex probes used in the study

From: Nanopore electric snapshots of an RNA tertiary folding pathway

Probe

Sequence

T2a

5ʹ-(CAT)10-gcugaccagcuaugaggucauacaucgucauagcac-(CAT)10-biotin-3ʹ

T2-PolyUb

5ʹ-(CAT)10-gcugaccagcuaugaggucauuuuuuuucauagcac-(CAT)10-biotin-3ʹ

Ref-HPc

5ʹ-(CAT)10-gcugaccucauagcuuuuuuugcuaugagguca-(CAT)10-biotin-3ʹ

  1. aT2 RNA forms a pseudoknot as shown in Fig. 1a
  2. bT2-PolyU RNA is the same as T2 RNA except that loop L2 (uacaucg) is substituted with a 7-nt polyU fragment (Fig. 5a)
  3. cReference hairpin forms a 12-bp joint helix of H1 and H2, and a 7-nt polyU loop (Supplementary Fig. 2a)
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