Fig. 3
From: Determining therapeutic susceptibility in multiple myeloma by single-cell mass accumulation
MAR defines drug sensitivity of human multiple myeloma ANBL-6 and MM.1 cells to bortezomib-dexamethasone combination. a, b MAR per mass of ANBL-6 and MM.1 cells treated in dimethyl sulfoxide (DMSO), 5 nM bortezomib, 200 nM dexamethasone and their combinations for 3 h. MAR per mass of ANBL-6.WT cells (bortezomib and dexamethasone sensitive) reduces upon the exposure to bortezomib and dexamethasone, while that of ANBL-6.BR cells (bortezomib resistant and dexamethasone sensitive) reduces only upon the exposure to treatment containing dexamethasone. MAR per mass of MM.1S cells (bortezomib and dexamethasone sensitive) reduces upon the exposure to bortezomib and dexamethasone, while that of MM.1R cells (dexamethasone resistant and bortezomib sensitive) reduces only upon the exposure to treatment containing bortezomib. Boxes represent the inter-quartile range and white squares are the average of all measurements. p values were calculated using Welch’s t-test, comparing treated cells to control (cells seeded only in culture media), and were Bonferroni corrected. ****p < 10−4 in highlighted segments. For ANBL-6.WT cells; p(DMSO vs. DMSO + dex.) = 3.2 × 10−11, p(control vs. bort.) = 1.5 × 10−14, p(DMSO vs. DMSO + dex. + bort.) = 3.7 × 10−38, for ANBL-6.BR cells; p(DMSO vs. DMSO + dex.) = 4.2 × 10−6, p(control vs. bort.) = 1, p(DMSO vs. DMSO + dex. + bort.) = 4.5 × 10−4, for MM.1S cells; p(DMSO vs. DMSO + dex.) = 7.6 × 10−6, p(control vs. bort.) = 6.3 × 10−7, p(DMSO vs. DMSO + dex. + bort.) = 2.3 × 10−31 and for MM.1 R cells, p(DMSO vs. DMSO + dex.) = 1, p(control vs. bort.) = 6.6 × 10−52, p(DMSO vs. DMSO + dex. + bort.) = 1.0 × 10−44. The number of cells in MAR measurements from left to right; n(ANBL-6.WT) = 61, 67, 63, 59, 64 and n(ANBL-6.BR) = 64, 59, 65, 68, 60 and n(MM.1S) = 60, 61, 64, 58, 59 and n(MM1.R) = 65, 61, 60, 64, 58. c, d ROC curves of control and treatment groups (DMSO, DMSO + dex, bort, DMSO + dex + bort) for ANBL-6 and MM.1 cells. e, f Cell viability analysis for ANBL-6 and MM.1 cells under different drug combinations
