Fig. 7
From: A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish
CRISPR-mediated disruption of tmc2a and tmc2b. Graphical representation of the tmc2a a and tmc2b f loci in zebrafish. Putative exons and splice sites are displayed. Red arrows mark the targeted exons. Segments of tmc2a exon 6 (b) tmc2b exon 7 (g) were subjected to genome editing. Engineered CRISPR sgRNAs bind target sites to enable DNA cleavage. Mutagenesis deleted 2 nucleotides of tmc2a (b) and deleted 5 nucleotides of tmc2b (g), yielding frame-shift mutations. c, h Amino acid sequences of wild-type and mutant proteins. Sequencing results of mutagenized and control loci, from tmc2a (d) and tmc2b (i). Blue highlight and blue delta indicate deleted nucleotides in mutants. Red highlight denotes the stop codons that were generated near the CRISPR-targeting site. e, j (top) Topographical representations of the Tmc2a (e) and Tmc2b (j) proteins. Arrowheads indicate points of introduced mutations. Amino acids of transmembrane domains are labeled in blue and the TMC domains are in green. (bottom) Schematics of predicted truncated polypeptides produced in double mutant are displayed
