Fig. 2

3D label-free mPAM image of an unstained mouse brain embedded in a paraffin block. a A section of the entire mouse brain image (coronal view). b A 3D view of the imaged brain block corresponding to the marked region in a. c x–y image at z = 0.16 mm (coronal view), with the cell nuclei marked in blue. The yellow dashed line outlines the boundary between the cerebrum and the cerebellum. d x–z image at y = 2.31 mm (transverse view). e y–z image at x = 0.63 mm (sagittal view)