Fig. 3
3D label-free mPAM image of an unstained mouse brain embedded in an agarose block. a An mPAM volumetric image of a mouse brain with a sectioning step size of 200 µm. The bottom right yellow dashed inset shows the corresponding projection and its imaging area (yellow shaded region) of a 3D mouse brain model (reprinted from Elsevier, Vol. 53, Johnson, G.A., Badea, A., Brandenburg, J., Cofer, G., Fubara, B., Liu, S. & Nissanov, J., Waxholm space: an image-based reference for coordinating mouse brain research, 365–372, copyright (2011), with permission from Elsevier)33. Three features, namely the lateral ventricles, hippocampus, and cerebellum, are labeled on the 3D mPAM image. b–k The relative positions of ten coronal views are labeled in a, and they are shown individually. The separation between each section is 500 µm
