Fig. 1
From: O-GlcNAcylation of SIRT1 enhances its deacetylase activity and promotes cytoprotection under stress
OGT interacts with SIRT1 in vivo and in vitro. a NCI-H1299 whole-cell extracts (Input) and immunoprecipitates of anti-SIRT1 antibody and control IgG were analyzed by IB, with the SIRT1-silenced NCI-H1299 cells used as a negative control. Data represent two independent experiments. b CoIPs by anti-OGT antibody from either control or OGT-silenced NCI-H1299 whole-cell extracts were analyzed by IB. Data represent two independent experiments. c Co-localization of endogenous OGT (Alexa Fluor 594) and SIRT1 (Alexa Fluor 488) was determined by immunofluorescence in NCI-H1299 cells. DAPI staining was used to identify the nucleus. Scale bars: 20 µm. d Direct interaction of SIRT1 with GST-OGT in an in vitro GST pull-down assay. Data represent two independent experiments. e Mapping of the SIRT1 domain responsible for the interaction with OGT. GST pull-down assays were used to determine the interaction of OGT with GST-SIRT1 deletion mutants. Data represent two independent experiments
