Fig. 4

De novo production of pleuromutilin intermediates. a Proposed biosynthetic pathway to 1 in Clitopilus passeckerianus. b HPLC chromatograms showing de novo production of pleuromutilin intermediates in Aspergillus oryzae strains. (i) A. oryzae GCP1, (ii) A. oryzae GCP1P2, (iii) A. oryzae GCP1P2S, (iv) A. oryzae GCP1P2SA and (v) A. oryzae NSAR1 (recipient strain). Traces were recorded with evaporative light scattering detector (ELSD)