Fig. 3
From: Atomic resolution snapshot of Leishmania ribosome inhibition by the aminoglycoside paromomycin
RNA modifications reveal an unexpected pattern of mixed bacterial and eukaryotic origins. a The high-resolution structure obtained in this study enabled the direct visualization of rRNA modifications in the leishmanial ribosome, many of which are localized at the decoding center (circled) in close proximity to the PAR binding pocket. 2′-O-methylated residues are highlighted in green, pseudouridines in magenta and unique base modifications are colored blue. b The PAR-binding pocket at the leishmanial ribosome is decorated with modified residues that are of mixed bacterial and eukaryotic origin. Modifications of bacterial origin (light blue) at h44 of SSU are clustered with two universally conserved modifications at h45 (magenta), to form a bridge that connects elements of the decoding center with mRNA A-site codon (yellow). H69 (light green) of LSU is also heavily modified and penetrates the PAR binding pocket. Leishmania H69 modifications of eukaryotic origin are highlighted in green, and universally conserved modifications in magenta. c rRNA modifications in the binding pocket. Densities are shown at 3.0 σ. Modified moieties are indicated by arrow and highlighted in yellow
