Fig. 4
From: The natural product carolacton inhibits folate-dependent C1 metabolism by targeting FolD/MTHFD
Carolacton effects on the human enzyme hsMTHFD2. a IC50 determination for carolacton against DH and CYH activity of hsMTHFD2. Data are presented as means ± s.e.m. of three independent replicates. IC50s were obtained via logistic dose–response fitting. The one-way ANOVA test was used for statistical analysis, P < 0.01. b SPR analysis of carolacton binding to hsMTHFD2. Carolacton shows strong binding to hsMTHFD2. The estimated K D was obtained by fitting the association and dissociation signals with a 1:1 interacton model using the Biacore X 100 Evaluation Software
