Fig. 1
From: Development of a genetic sensor that eliminates p53 deficient cells
Development of a genetic p53 sensor. a Relative luciferase expression is shown for three p53-repressed elements (RE) derived from CDC25C, SCD, and RGS13 promoters. b Relative luciferase expression is shown for four different fragments (F) derived from SCD promoter and/or 5′UTR/intron1. c Relative luciferase expression is shown for four p53-activated elements (AE) derived from p21 and PUMA promoters. d Combination of repressed and activated elements. Relative luciferase expression is given for cotransfection of indicated constructs. a, b, c, d The activation/repression folds were calculated between pCMV and pCMV-p53wt cotransfections and are given as numbers above the bars. All error bars represent the standard deviation (SD) of three independent experiments and Student’s two-tailed t-test values are represented comparing expression between pCMV and pCMV-p53wt cotransfections (***P < 0.001, **P < 0.01, *P < 0.05; ns, not significant; SRE, sterol regulatory element; CGIs, CpG islands)
