Fig. 4

Design and functional characterization of Chimera. a Subunit structure of wild-type PriSLX and Chimera. In the second subunit of Chimera, amino acids 1 to 211 of PriL are fused to amino acids 42 to 154 of PriX. b Primer synthesis assay on mixed-sequence ssDNA 30mer with fixed concentration of wild-type and mutant primase, at 10′, 20′ and 40′ time points. The reaction products were separated by denaturing PAGE, stained with SYBR Gold and detected by phosphorimaging. c Primer synthesis assay on poly(dT) ssDNA with increasing concentrations (125 nM, 250 nM, and 500 nM) of wild-type PriSLX and Chimera. The reaction products were separated by PAGE under denaturing conditions and detected by phosphorimaging of the incorporated ³²P-αATP