Fig. 5
From: Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster
DNA replication assays. Relative levels of DNA synthesis were measured in a reconstituted DNA replication assay with purified proteins on a M13 ssDNA template, in the presence of wild-type primase (control) and Chimera (a) or wild-type primase (control) and single-point mutants R232A, D70A, R72A and R74A (b). The reactions contain 3.125 nM, 6.25 nM, 12.5 nM, 25 nM, or 50 nM primases. The reaction products were separated by alkaline agarose gel electrophoresis and detected by phosphorimaging of the incorporated 32P-αdCTP
