Fig. 7
From: Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster
Structure-based cross-linking of PriSLX. a Ribbon diagram of PriSLX. The side chains of PriS E34 and PriX K110, that were targeted for cysteine mutagenesis, are drawn in stick representation at the PriS–PriX interface. The side chain of PriS T11, that was used as control in the cross-linking reactions, is also shown. b BMOE cross-linking of PriSLX and Chimera PriS E34C, PriX K110C double mutants. The PriS T11C, E34C and PriS T11C, PriX K110C double mutants were used as controls. The products of the cross-linking reactions were separated by SDS-PAGE and stained with Coomassie Blue. The arrowheads mark the product of the specific cross-linking between PriS E34 and PriX K110
