Fig. 8

Plp1-Cre/ERT–mediated Trpa1 deletion from Schwann cells prevented the partial sciatic nerve ligation (pSNL)-evoked allodynia and neuroinflammation. a Acute nociception response induced by intraplantar AITC (10 nmol 20 µl⁻¹) or vehicle (veh, 0.5% DMSO) in Plp1-Cre ^ERT ;Trpa1 ^fl/fl and control mice (n = 6, ***P < 0.001 AITC vs. veh; one-way ANOVA followed by Bonferroni post hoc analyses). b Triple immunofluorescence staining and colocalization value (Rcoloc) of TRPA1, PGP9.5, and S100 (Scale bars: 20 µm) in sciatic nerve trunks obtained from Plp1-Cre ^ERT ;Trpa1 ^fl/fl (n = 5) and control mice (n = 4), (**P < 0.01; Plp1-Cre ^ERT ;Trpa1 ^fl/fl vs. control; unpaired two-tailed Student’s t-test). c Ca²⁺ responses to AITC (1 mM) or vehicle (veh, 1% DMSO) in cultured Schwann cells from sciatic nerve trunks of Plp1-Cre ^ERT ;Trpa1 ^fl/fl and control mice (n = 25 cells/two independent experiments, ***P < 0.001 AITC vs. veh; ^§§§ P < 0.001 AITC Plp1-Cre ^ERT ;Trpa1 ^fl/fl vs. AITC control; one-way ANOVA followed by Bonferroni post hoc analyses). d Mechanical allodynia, and (e) representative images, F4/80⁺ cells, and H₂O₂ content (at day 10 after surgery) in sham/pSNL Plp1-Cre ^ERT ;Trpa1 ^fl/fl and control mice (n = 8, *P < 0.05 and ***P < 0.001 pSNL control vs. sham control; ^§§ P < 0.01 and ^§§§ P < 0.001 pSNL Plp1-Cre ^ERT ;Trpa1 ^fl/fl vs. pSNL control; (d) two-way ANOVA followed by Bonferroni post hoc analyses and (e) one-way ANOVA followed by Bonferroni post hoc analyses) (Scale bars: 50 μm). Data are represented as mean ± s.e.m