Fig. 2
CSDE1 protein levels decrease with differentiation in developing mouse embryonic tissues. a Western blot analysis with antibody to CSDE1 of mouse ESCs (mESCs) and derived NPCs. The graph represents the CSDE1 relative percentage values (corrected for GAPDH loading control) to mESCs (mean ± s.e.m. of two independent experiments). b Immunocytochemistry of mESCs and differentiated cells with antibody to CSDE1. NANOG and DAPI staining were used as markers of pluripotency and nuclei, respectively. Scale bar represents 20 μm. c Western blot analysis of primitive streak and streak-enriched tissues (posterior) and neural plate and neural-enriched tissues (anterior) from mouse embryos (embryonic day (E)8.5)). T (brachyury) and SOX2 staining were used as markers of posterior and anterior parts, respectively. The graph represents the CSDE1 relative percentage values (corrected for GAPDH loading control) to posterior part (mean ± s.e.m. of three independent experiments). All the statistical comparisons were made by Student’s t-test for unpaired samples. P-value: *(P < 0.05), **** (P < 0.0001)
