Fig. 4
Loss of CSDE1 promotes neural differentiation and neurogenesis. a Percentage of OCT4 and PAX6-positive cells/total nuclei at different days after neural induction of undifferentiated H9 hESC colonies (mean ± s.e.m. of three independent experiments, 200 total cells per experiment). b qPCR analysis after 10 days of neural induction. Data (relative expression to NT shRNA cells) represent two independent experiments with three biological replicates. c qPCR analysis of neuronal markers after 10 days of pan-neuronal differentiation. Graph (relative expression to NT shRNA) represents the mean ± s.e.m. of three independent experiments with three biological replicates. d Immunocytochemistry after 7 and 21 days of neuronal induction. MAP2, GFAP, and Hoechst staining were used as markers of neurons, astrocytes, and nuclei, respectively. Scale bar represents 20 μm. e qPCR analysis of endoderm markers upon definitive endodermal differentiation of undifferentiated H9 hESCs. Graph (relative expression to NT shRNA) represents the mean ± s.e.m. of three independent experiments with two biological replicates. All the statistical comparisons were made by Student’s t-test for unpaired samples. P-value: *(P < 0.05), **(P < 0.01), ***(P < 0.001), **** (P < 0.0001)
