Fig. 6
Loss of FABP7 in hESCs reduces their neural differentiation potential. a qPCR analysis in FABP7 and VIM KD H9 hESCs. Graph (relative expression to NT shRNA H9 hESCs) represents the mean ± s.e.m. of two independent experiments with three biological replicates. b Knockdown of FABP7 slows down neural differentiation. Percentage of OCT4 and PAX6-positive cells/total nuclei at different days after neural induction of H9 hESCs (mean ± s.e.m. (n = 3, 400–650 total cells per data point). c After 10 days of neural induction, cells were assessed by immunofluorescence with OCT4, PAX6, and Hoechst staining. Scale bar represents 20 μm. d Quantification of the percentage of PAX6-positive cells/total nuclei after 10 days of neural induction. Graph represents the mean ± s.e.m. of 3 independent experiments, 3000 total cells per experiment. e qPCR analysis after 10 days of neural induction. Graph (relative expression to NT shRNA cells) represents the mean ± s.e.m. of three independent experiments. f qPCR analysis after 3 weeks of neuronal induction. Data (relative expression to NT shRNA cells) represent the mean ± s.e.m. of three independent experiments with three biological replicates. g After 3 weeks of neuronal induction, cells were assessed by immunofluorescence with MAP2, GFAP, and Hoechst staining. Scale bar represents 20 μm. All the statistical comparisons were made by Student’s t-test for unpaired samples. P-value: *(P < 0.05), **(P < 0.01), ***(P < 0.001), **** (P < 0.0001)
