Table 1 Cryo-EM data collection and refinement statistics

From: Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes

Human i-20S-PKS21004 (EMD-7010, PDB 6AVO)

Data collection

EM equipment

JEOL JEM-3200FS

Voltage (kV)

300

Detector

Gatan K2 Summit

Pixel Size (Å)

1.2

Electron Dose (e− Å−2)

60

Underfocus range (µm)

1.0–3.0

Reconstruction

Software

RELION 2.0

Number of particles used

75,017

Resolution (Å)

3.8

Map-sharpening B factor (Å2)

124.3

Model composition

Peptide chains

28

Protein residues

6115

Ligands

2

R.m.s deviation

Bond length

0.009

Bond angle

1.146

Ramachandran plot

Preferred (%)

90.70

Allowed (%)

9.22

Outlier (%)

0.08

Validation

MolProbity score

2.52

Good rotamer (%)

98.02

Clashscore, all atoms

32.47

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