Fig. 2

Hi-C data reveal translocation events. a Fifty-six inter-chromosomal translocation events identified in RPMI-8226 by WGS. From outer circle to inner circle: panel I: average RNA-seq count for every 200 kb chromosome bin; panel II: CNV data from WGS (black dots: copy number equal to 2, red dots: copy numbers larger than 2, green dots: copy numbers less than 2); panel III: orange lines: translocation events, blue lines: translocation events that are among the top 100 highest inter-chromosomal Hi-C interactions in b. b The 100 highest inter-chromosomal Hi-C interactions in RPMI-8226 cells (5 M resolution): from outer circle to inner circle: panel I and II: the same as in a for RNA-seq counts and CNV data; panel III: orange lines: top 100 highest interactions, blue lines: the common events with a. c The chimeric chromosome formed between chr16 and chr22 identified by spectral karyotyping (SKY). The SKY image of RPMI-8226 is from the NCBI SKY database. d The translocation event of t(16;22)(q23.2;q11.22) in RPMI-8226 cells plotted by IGV tool, showing only WGS paired reads supporting this translocation event. The reads depth and alignment panels are shown. Read alignments are sorted by read location and colored by read strands. e Top panel: the intra and inter-chromosome interaction maps of chromosome 16 and 22 in RPMI-8226. Bottom panel: copy number variations of chromosome 16 and 22. f Enlarged inter-chromosomal interactions corresponding to the translocation t(16;22)(q23.2;q11.22)