Fig. 4
From: The HSF1–PARP13–PARP1 complex facilitates DNA repair and promotes mammary tumorigenesis
HSF1–PARP13 promotes PARP1 redistribution and chromatin opening. a Schematic view of HSEs in the promoter of the GADD34 gene. Bars indicate amplified DNA regions by ChIP-qPCR. Exons and HSE sequences are shown. Consensus nGAAn units are underlined and conserved sequences are highlighted in red. b Constitutive occupancy of HSF1, PARP1, and PARP13 on the HSEs. ChIP-qPCR was performed after each knockdown (n = 3). Double knockdown (DKD) indicates knockdown of both PARP1 and PARP13. c Occupancy of PARP1 and PAR on the GADD34 locus in cells expressing each hHSF1 mutant. ChIP-qPCR on the HSE and each gene locus (region 1 or 2) was performed before and after DOX treatment (n = 3). d Occupancy of PARP1 and PAR on the GADD34 locus in cells expressing each hPARP1 mutant. ChIP-qPCR was performed before and after DOX treatment (n = 3). e Levels of active chromatin marks on the GADD34 locus in cells expressing each hHSF1 mutant. ChIP-qPCR was performed before and after DOX treatment (n = 3). Mean ± s.d. is shown. Asterisks indicate P < 0.01 by Student’s t-test
