Fig. 1

Identification of polyketides from wild-type extracts. a Results of XCMS analysis of wild-type C. acetobutylicum and Δpks extracts taken from early stationary phase fermentation cultures (biological quadruplicates). Blue (upper) chromatograms depict MS traces of quadruplicate wild-type extracts, while orange/brown (lower) chromatograms depict MS traces of quadruplicate Δpks extracts. Green circles represent MS peaks unique to wild-type extracts, with corresponding m/z values indicated by the y-axis. No significant MS peaks were identified, which were unique to Δpks. The three peaks unique to wild type are identified as 1, 2, and 3. b UV–Vis traces (240 nm) of extracts from wild type, Δpks, and Δpks genetic complementation strain (Δpks (pCKO_pks)). c Elucidated structures of 1 and 3 based on NMR characterization