Fig. 8
From: Carrageenan catabolism is encoded by a complex regulon in marine heterotrophic bacteria
Affinity gel electrophoresis for the SusD-like proteins and phenotyping for the susCD-like mutant ΔcgtA-cgtB. a BSA and recombinant ZGAL_3580 (CgtB, SusD-like) and ZGAL_3638 (SusD-like) migrated at 60 V over 5 h in native-PAGE gels or in gels containing different red algal cell wall polysaccharides. The delay of migration for ZGAL_3580 (red line) and ZGAL_3638 (green line) was compared between the native gels and the polysaccharide containing gels by comparison with BSA (black line). b Growth curves for wild-type Z. galactanivorans (black curves) and ΔcgtA-cgtB (red curves) in rich Zobell media and in minimal media containing different carbohydrate substrates
