Fig. 1
From: The genetic basis for the adaptation of E. coli to sugar synthesis from CO2
Finding a compact sufficient set of mutations from the evolved allele pools. a Hemiautotrophic growth, in which CO2 is fixed for sugar synthesis, occurred independently in three chemostat evolutions (green circles, numbers indicate the number of extra mutations not written). Integration of the prs and ppsR mutations into ancestral strain and “replaying” evolution yielded a much more compact set of mutations (light green) (Antonovsky et al.)3. b The evolved alleles were combinatorially introduced to find a sufficient set which is locally minimal, i.e., removal of any mutation from the set will cause loss of the phenotype. The reconstructed strain was evaluated and characterized by suppressor analysis to understand the functional role of each mutation
