Fig. 2
From: The genetic basis for the adaptation of E. coli to sugar synthesis from CO2
A locally minimal set of mutations enables hemiautotrophic growth. a Role of mutated genes (red) in central carbon metabolism. Three mutations are located in branch points of the synthetic CBB cycle (serA;H210Q, pgi;G378C, and prs;R105_A110dup). Two mutations are regulators influencing the carbon metabolism (crp;M190K and ΔppsR). The initial strain design decouples central carbon metabolism in order to create two distinct modules, one for energy production and second for CO2 fixation. In green, biomass components originating from CO2; in blue, end products of pyruvate supply. Ovals mark the feed substrates. b The reconstructed strain has five essential mutations that enable CO2 fixation. Reverting every mutation independently back to WT prevents hemiautotrophic growth on liquid media, showing that this set of mutations is sufficient and irreducible
