Fig. 4 | Nature Communications

Fig. 4

From: Inference of differentiation time for single cell transcriptomes using cell population reference data

Fig. 4

Regulation of neural differentiation timing and the cell cycle by Fyn. a Expression levels of Fyn, differentiation markers Sox1, Jag1, and Hey1 and M-phase markers Eb1/Mapre1 in wild-type (WT) and Fyn KO E14 cells from days 0 to 6 of neural differentiation. Error bars indicate standard deviation of three technical replicates. b RNA-seq of WT and Fyn KO E14 cells revealed a significant downregulation of Fyn-co-expressed Notch pathway genes and M/G1-phase genes (genes in Fig. 3f, g) on day 4 of mESC neural differentiation. c Expression levels of Sox1 and Eb1/Mapre1, with or without G2/M arrest inhibitor PF477736 treatment from days 2 to 4 of neural differentiation. Error bars indicate standard deviations of three technical replicates

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